Polarization microscope by single polarization modulation method: Novel instrument for visualizing cytoskeleton
2009
A novel and simplest polarization microscope by single polarization modulation method to visualize cytoskeletal
structure in bio-cells have been developed. The polarization microscope visualizes cytoskeletal structure based on
birefringence characteristics of cells. In the polarization microscope, a series of intensity images according to single
modulation of rotating linear polarizer were obtained to visualize cytoskeletal structure. Phase retardation and principal
axis orientation were calculated by analytic data processing from the acquired intensity images. Phase retardation and
principal axis orientation of breast cancer cells, MCF 7 and MDA MB 231 were measured and visualized as their
birefringence characteristics by the polarization microscope. Phase retardation sensitivity of the polarization microscope
system also was measured in this paper.
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