Study of the impact of CLPTM1L on radiosensitivity of lung cancer

Objective To study the correlation of cleft lip and palate transmembrane 1 like (CLPTM1L) expression and radiosensitivity of lung cancer cells. Methods Thiazolyl blue tetrazolium bromide (MTT) and cell colony formation assays were used to determine cell growth and survival. Western Blot assay was employed to measure protein expression. Results The results demonstrated a negative correlation between the CLPTM1L expression level and radiosensitivity of lung cancer cells. A lower radiosensitivity in lung cancer cells containing high level of CLPTM1L expression, and vice versa. Enforced expression of CLPTM1L resulted in a significant reduction of radiosensitivity in lung cancer cells irradiated with γ-rays. On the contrary, a marked elevation of radiosensitivity was observed in lung cancer cells transfected with CLPTM1L siRNA. Conclusions CLPTM1L may be a novel target gene in mediating radiosensitivity of lung cancer cells. Key words: Cleft lip and palate transmembrane 1 like; Radiation tolerance; Lung neoplasms; γ-rays