MP68-04 A FIVE—GENE DNA—METHYLATION BIOMARKER PANEL SENSITIVELY DETECTS BLADDER CANCER AND DISCRIMINATES BETWEEN HIGH—GRADE AND LOW—GRADE DISEASE IN VOIDED URINE

RESULTS: Our series of patients included 39 BCG-successful and 20 BCG-failure patients. The expression levels of OCT3/4 in the BCG non-responders were significantly higher than those of the BCG responders. In vitro study UM-UC3 showed a strong resistance to BCG treatment than T24. The expression levels of CSLCs-related genes (OCT3/4, Nanog, and SOX2) and EMT-related genes (Snail) increased after BCG treatment in UM-UC3 whereas T24 showed the contrary results. In sphere formation assay UM-UC3 showed a higher colonization ability than T24 after BCG treatment. CONCLUSIONS: Higher expression levels of CSLCsand EMT-related genes reflected BCG failure. The population of CSLCs in the primary CIS may be one of the predictors for BCG failure.