302 varieties of one kind Taxodium'zhongshansha tissue culture propagation method

The present invention discloses a method for breeding varieties Taxodium'zhongshansha 302 tissue culture, the method comprising the steps of: (1) Select the explants and disinfection; (2) be modified original WPM medium; (3) the process after the explants cutting, for the first generation of induced culture stem segments; (4) the step (3) obtained in shoot for subculture; for rooting (5) in step (4) is obtained without roots plantlets culture; aseptic culture acclimation hardening (6) obtained in step (5). With this method Taxodium'zhongshansha breeding nursery 302, 102-110 fold proliferation rate, and the breeding time is short.