郏县红牛POU1F1基因第6外显子Hinf I、Alu I和Pst I位点遗传变异及其与生长发育的关系

[Objective] The objective of the present study was to study the genetic effects of polymorphisms of HinfⅠ, AluⅠ and PstⅠ loci within POU1F1 gene exon 6 on Jianxian Red cattle growth traits. [Method] POU1F1 gene exon 6 polymorphic fragments were detected by PCR-RFLP technique using 144 Jianxian Red cattle. Association analysis of POU1F1 gene exon 6 polymorphism with growth traits was also conducted. [Result] The results showed that 451 bp PCR products digested with HinfⅠ demonstrated polymorphisms. Moreover, the frequencies of allele AA, AB and BB genotype of Jiaxian red cattle were 0.1875, 0.3056 and 0.5069, accordingly, the frequencies of allele A and B were 0.340 3 and 0.659 7 respectively. The homozygositiy, heterozytosiy, the effective allele numbers, and PIC value of POU1F1 gene exon 6 HinfⅠ locus were 0.5510, 0.4490, 1.8148 and 0.3482 respectively. As this locus belonged to the middle polymorphic index, it was suggested to be an effective evaluation parameter for studying genetic resource However,no polymorphism was found at POU1F1 gene exon 6 AluⅠ and Pst I loci. The correlation of HinfⅠ polymorphisms of POU1F1 gene exon 6 and growth traits was analyzed and the result showed that there was significant relationship between Hinf I polymorphism and body length (P＜0.05). The individual with genotype BB had longer body length than the individual with genotype AB and AA, suggesting the allele B had significant effect on body length of Jianxian red cattle. [Conclusion] The genotype BB was significantly correlated with the fine performance and the selection of the allele B was better for the improvement.