Method for preparing humanized immune globulin capable of antagonizing vessel endothelium cell growth factor and combined uses thereof

The present invention relates to biotechnology, belongs to the field of recombinant gene engineering, and is six kinds of humanized fused immunoglobulins prepared through recombinant gene engineering and cell culturing process and capable of recognizing and combining with vascular endothelial cell growth factor specifically and their application. These six kinds of fused immunoglobulins feature that each of them has immunoglobulin-like structure regions capable of being combined with VEGF in the N-end and human immunoglobulin constant region in the C-end. These fused immunoglobulins have the bioactivity of high combination with VEGF and the bioactivity of immunoglobulin constant region, and can neutralize the VEGF inside adsorbed body to antagonize and inhibit fission and proliferation of vascular endothelial cell. These fused immunoglobulins are applied as VEGF neutralizing adsorbent alone or in combination.