Microscopic detection of Fasciola hepatica in drinking water sources of district Dera Ismail Khan, KP, Pakistan

A study was conducted to determine the presence of zoonotic parasite (Fasciola hepatica) in drinking water sources of five different areas madina colony, islamiacolony, deyal road, zafrabad colony and dikhan city in district D.I. Khan, Khyber Pakhtunkhwa and their zoonotic potential. A total of 200 water samples were collected from the three different sources such as tap water, pond water, drain water. The water samples were filtered through Whatt-man filter paper (USA) in water filtration assembly and the residual solid particles containing parasites were collected from the filter paper. The filtered residue was centrifuged at 4000 rpm for 5 minutes, the supernatant was discarded and the pallet was preserved in an eppendorf tubes and stored at 4 °C for further process. In the present study, overall prevalence was 5% (10/200). The presence of Fasiola hepaticain tap water was 1% (1/100), in pond water was 6% (3/50) and in drain water was 12% (6/50).