Possibilities of differentiation of the specific positive reactions for pullorum disease

Abstract Studied were sera obtained from three groups of birds experimentally infected with Salmonella gallinarum-pullorum. Salmonella enteritidis, and Salmonella heidelberg, resp., using the whole serum agglutination reaction, the slow test-tube reaction (at 37 degrees and 50 degrees C), and the agar gel precipitation reaction as modified by Aoki as well as the spot-test of Williams. It was found that with the use of a typhus antigen it is not possible to differentiate the positive coagglutination reactions between Salmonella gallinarum-pullorum and the Salmonella bacteria of the B and D serologic groups. Differentiation of the coagglutination reactions between Salmonella gallinarum-pullorum and the bacteria of B serologic Salmonella group was effected through exhaustion of the sera with Salmonella-gallinarum pullorum after Castellani, followed by the study with an O-test antigen of Salmonella heidelberg.