Enzymatic Δ1-Dehydrogenation of 3-Ketosteroids—Reconciliation of Kinetic Isotope Effects with the Reaction Mechanism

The authors acknowledge partial financial support from the National Science Centre Poland under the OPUS grant number UMO-2016/21/B/ST4/03798. M.G and P.W. acknowledge the fellowship with project no. POWR. 03.02.00-00-I013/16. This research was supported in part by PLGrid (CYFRONET) Infrastructure, by the Spanish Ministerio de Ciencia e Innovacion (grant PGC2018-094852-B-C21 and PID2019-107098RJ-I00), the Generalitat Valenciana (grant AICO/2019/195 and SEJI/2020/007) and Universitat Jaume I (grant UJI·B2020-031 and UJI-A2019-04). We acknowledge the joint consortium “Interdisciplinary Centre of Physical, Chemical and Biological Sciences” of ICSC PAS and INP PAS for providing access to the Agilent 1290 Infinity System with an automatic autosampler and an M.S. Agilent 6460 Triple Quad Detector.