Generation and Application of Monoclonal Antibodies against Salmon Somatotropin (Salmon Growth Hormone) and Salmon Prolactin

Hybridomas that produce two monoclonal antibodies (MoAbs) to salmon somatotropin (sST) t and two MoAbs to salmon prolactin (sPRL) were established. Two MoAbs to sST, designated KM 166 and KM 167, reacted specifically with sST and did not react with sPRL, proteins derived from host Escherichia coli, or bovine serum albumin. KM200 and KM212 raised against sPRL were specific for sPRL. Using these MoAbs we have developed a sandwich-type enzyme immunoassay (ELISA) system for sST or sPRL. With these assays, sST and sPRL could be quantitatively n measured in the range of 0.16~4μg/ml and 3.1 ~200μg/ml, respectively. The immunoaffinity column using KM200 adsorbed specifically sPRL. By elution with 7 m urea/1 m NaCl, the sPRL was eluted with a high recovery.