Digital Microscopy Technologies: A Method for Constructing Incision Lines on Cell Conglomerate Images

The article is devoted to the technology of separating conglomerates of leukocyte cells in digital microscopy images of bone marrow preparations in the light range for determining the number of cells and automatic diagnosing. A special feature of the proposed method is the use of the principle of separating cell conglomerates based on abnormal points. The key points of the method: the determination of the cell conglomerate contour, the detection of abnormal points, and sectioning the cell conglomerate image by the bisector of the angle whose vertex is the abnormal point. The parameters providing necessary accuracy of cell conglomerate separation are determined. Here the accuracy is understood as a fraction of correctly separated cells in the images of bone marrow preparations with respect to the total number of cells in the images used in the experiment. Images containing cell conglomerates forming chains were considered. The experiment confirmed the efficiency of the proposed method. The separation accuracy of adjacent cells is 95%.