Zinc clasp-based reversible toolset for selective metal-mediated protein heterodimerization
2018
Considering the complex biological quandaries of the tightly woven networks of biological macromolecules, we present an optimized zinc clasp-based toolset from the CD4 co-receptor and Lck protein tyrosine kinase complex for selective, tight and fully reversible protein heterodimerization (log K12 = 18.6). We demonstrated its utility on CD4-tagged proteins with capture from bacterial lysate and constructed molecular baits using a new small-molecule tether.
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
35
References
3
Citations
NaN
KQI