Analysis of Pregnancy-Induced Anti-HLA Antibodies Using Luminex Platform
2015
Abstract Background The impact of pregnancy on the incidence of HLA sensitization is poorly explored, and most of the data were obtained by use of a complement-dependent cytotoxicity (CDC) assay with an incidence of 18% to 30%. In many cases, antibodies cannot be detected by CDC as their level fades in time. However, this state of “latent” sensitization can still have a significant clinical impact in a subsequent transplantation setting. Methods Pregnancy-induced HLA sensitization was analyzed in 69 mothers without a history of transfusions or transplants. The mothers and their offspring were typed for HLA-A, HLA-B, and HLA-DR. Sera from the mothers were studied using the Luminex screening test to detect anti-HLA class I and class II antibodies. Positive and borderline sera were further analyzed using single antigen kits. Results Thirty-four sera (49.3%) were positive in the screening test (52.9% for class I and 61.7% for class II antibodies). All of them tested positive for paternal alloantigens by single antigen. Positive paternal alloantigens were also detected in 52.9% of class I and 36.3% of class II sera with borderline screening. Interestingly, in a further analysis, the mean of the MFIs of paternal alloantigens that resulted negative was calculated and compared with that of neutral alloantigens and that of maternal self-antigens; in both cases, differences were highly significant (288 vs 153, P P Conclusions This study shows a high incidence of pregnancy-induced HLA sensitization and suggests that HLA typing of partners of women who have been pregnant may contribute to a better risk assessment in the transplantation setting.
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