Phyto-therapeutic potential of stem bark of the wonder tree, Prosopis cineraria (L.) Druce in LPS-induced mouse model: An Anti-Inflammatory Study

Therapeutic potential of Prosopis cineraria has been extensively explored by many eminent researchers against various serious diseases but its activity against Lipopolysaccharide (endotoxin) is obscure. Therefore, present experimental investigation was conducted to unravel and analyze the anti-inflammatory potential of hydro-ethanol extract of Prosopis cineraria against LPS-induced inflammation in Swiss albino male mice. Swiss Albino male mice were intoxicated (intra-peritoneal) with LPS (2 mg/kg body weight) and further treated with low and high dose (i.e. 100 mg/kg body weight and 300 mg/kg body weight respectively) of hydro-ethanol extract of stem-bark of Prosopis cineraria. The levels of cytokines (TNF-α, Prostaglandins E2, IL-6, NF-κBp65, IFN-γ and IL-10) were determined in liver homogenate. Nitric oxide generated due to LPS-induced toxicity was estimated by using Griess reagent. The results demonstrated that the plant extract suppressed the over-expression and altered levels of cytokines due to LPS intoxication and restored the levels of TNF-α, NF-κB, NO, IL-6, IFN- γ, Prostaglandin E2 and IL-10. The present research work unravelled the alleviating potential of Prosopis cineraria against LPS-induced inflammation by modulating the expression of cytokines.