The molecular pathology examination analysis of K-ras gene mutation in colorectal cancer tissue

Objective: To investigate K-ras gene mutation and clinical pathological significance by the application of real- time quantitative PCR method. Methods: A total of 71 cases of colorectal cancer paraffin-embedded tissues were used real-time quantitative PCR assay to detect K-ras status. Results: The K-ras gene mutation rate was 35.22% (25/71) in colorectal cancer. Seven mutation types were found, namely Gly12Asp, Gly12Val, Gly12Cys, Gly12Ser, Gly12Ala, Gly12Arg and Gly13Asp, and one case of Gly12Val and Gly12Arg coexisting mutations were found. The 56% (14/25) mutations occur at the second base of codon 12, and the most common type was Gly12Asp. The mutation rate of the K-ras gene in the male group was lower than that in the female group, and the