DEVELOPMENT OF NANOSTRUCTURED LIPID CARRIER FORMULATION CONTAINING OF RETINYL PALMITATE

Objective: The purpose of this research is to develop a Nanostructure Lipid Carrier (NLC) formulation containing of Retinyl Palmitate. Methods: Preparation of NLC was carried out by ultrasonication method. The formulas of NLC were developed by using virgin coconut oil and oleic acid as a liquid lipids, cetyl palmitate, and stearic acid as solid lipids, Tween 80 and Poloxamer as a surfactant and glycerine as co-surfactant. Characterization of NLC consisted of visual appearance, morphology, particle size, polydispersity index (PI), and physical stability test using freeze-thaw, centrifugation, and accelerate stability test method. Results: Obtained NLC revealed a good characterization with the formulation of 7.2% of cetyl palmitate, 4.8% of oleic acid, 10% of Tween 80, 10% of glycerin, and 2% of retinyl palmitate. This NLC has a pale yellow appearance, globule size of 258±15.85 nm; and a polydispersity index of 0.31±0.09. It was also physically stable after centrifuged at 13,000 rpm for 30 min, during 4 cycles of freeze-thaw and storage at room temperature for 28 d. During storage for 28 d, retinyl palmitate in NLC had been degraded only about 15% in comparison with macroemulsion destroyed almost 50% of retinyl palmitate in the same time. Conclusion: NLC formulations with 7.2% cetyl palmitate, 4.8% of oleic acid, 10% of Tween 80, 10% of glycerol, and 2% of retinyl palmitate is the most optimal formula that showed a good characteristic. Stability study revealed that NLC provided better stability than macroemulsion. Keywords: Retinyl palmitate, NLC, Ultrasonication Methode