Randomized controlled study of the effect of a butter naturally enriched in trans fatty acids on blood lipids in healthy women

Background: Whereas the negative effect of consuming trans fatty acids found in partially hydrogenated vegetable oils on cardiovascular disease (CVD) risk is well established, the effect of trans fatty acids from ruminant sources (rTFAs) on CVD risk factors has not yet been established, particularly among women. Objective: We investigated the effects of a butter naturally enriched in rTFAs, of which vaccenic acid is the predominant isomer, on plasma lipid concentrations among healthy women. Design: In a double-blind, randomized, crossover controlled study, 61 healthy women aged 19–70 y were fed 2 isoenergetic diets lasting 4 wk each. The 2 diets were defined as moderately high in rTFAs (3.7 g/d, 1.5% of daily energy) and control (0.9 g/d, 0.3% of daily energy). Results: No significant effect of the rTFA diet was found on total plasma cholesterol, LDL cholesterol, apolipoprotein B, apolipoprotein A-I, and triglyceride concentrations compared with the control diet. There was a small yet statistically significant reduction in plasma HDL-cholesterol concentrations with the rTFA diet (22.8%; P = 0.004), which was significant (P for the BMI · treatment interaction = 0.006) among women with a BMI (in kg/m 2 ) 25 (25.2%; P = 0.004; n = 18) but not among women with a BMI ,25 (21.2%; P = 0.13; n = 43). Conclusions: These results suggest that an increase in dietary rTFAs equivalent to ;1% of daily energy has no significant effect on LDL but may be associated with a reduction in plasma HDLcholesterol concentrations, particularly in overweight women. This trial is registered at clinicaltrials.gov as NCT00930137. Am J Clin Nutr doi: 10.3945/ajcn.111.023408.