Generation of Protein In Situ Arrays by DiscernArray™ Technology

: A cell-free DiscernArray technology is described for making protein arrays directly from polymerase chain reaction deoxyribonucleic acid (PCR DNA) fragments. In this method, individual tagged proteins are synthesized in a cell-free system on a tag-binding surface, such that the tagged proteins are immobilized on the surface as they are produced. This technology is particularly useful for arraying proteins/domains that cannot be functionally produced by heterologous expression or for which the cloned DNA is not available. Protein arrays of single-chain antibodies, ligand-binding domains, and enzymes have been successfully generated by this method.