Retention reproducibility of basic drugs in high-performance liquid chromatography on a silica column with a methanol-ammonium nitrate eluent : Batch-to-batch reproducibility of the stationary phase

Abstract The effect of changing the brand and batches of the silica stationary phase used with a methanol-aqueous ammonium nitrate eluent for the high-performance liquid chromatographic separation of basic drugs has been studied. Considerable care had to be taken to obtain a reproducible eluent of small changes in the concentration of the ammonia solution were examined closely. Large differences in both the capacity factor and relative capacity factors were found for separations on columns packed with four different brands of silica. Significant differences were also observed with columns containing eighteen different batches of Spherisorb S5W, which had been manufactured over a period of several years.