Micropropagation of Mangifera indica L. cv. Kurakkan through somatic embryogenesis
2010
Nucellar embryogenesis was induced in Mangifera indica L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing rootstock. Nucellus tissue excised from 3.5 cm long fruits developed pro-embryonic callus in 19 days of inoculation on modified MS medium supplemented with 4.52µM 2,4D, 0.05% malt extract and 13.78µM spermidine. Somatic embryogenesis exhibited high frequency (158.33 embryos). However, all the differentiated embryos proliferated on medium having low level of sucrose (4% w/v) and auxin (2.26µM 2,4-D). Most of the proembryonic calli converted into heart shaped and cotyledonary embryos by reducing temperature to 15 o C. Somatic embryos were matured on modified MS medium fortified with 0.38µM ABA, 0.57µM IAA and 30.30µM PEG. Matured somatic embryos germinated (around 30%) on MS medium supplemented with 2.68µM NAA, 11.60µM kinetin and 2736.9µM glutamine.
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