Fertilization of mouse ova in vitro. I. Effect of some factors on fertilization.

Certain factors which could influence the process of fertilization in explanted ampullar parts of the oviduct were studied by a modification of the method of Brinster & Biggers (1965). The effects of homologous serum, glycine, lactate, glucose, pH and oxygen were tested, and also the fertilizing capacity of spermatozoa from the uterus 1 to 7 hr after copulation. The effect on fertilization of varying the concentration of non-agglutinated spermatozoa was evaluated. A de-