HLA-DQα PCR System: Frequencies of a South Bavarian Population and Family Data

The HLA-DQα locus and the analysis of amplified DNA by using non-radioactivelabelled oligonucleotide probes in dot-blot-form have first been described by Saiki et al. (1986). Further developement of the polymerase-chain-reaction followed (Saiki et al., 1988). Ultimately, Cetus Corporation, Emeryville, introduced it’s AmpliType® Type HLA-DQα kit (1989). The aim of our random test was to collect data about the distribution of HLA-DQα alleles and phenotypes in a South Bavarian population and to compare the obtained data with other statistics already described in relevant literature. Furthermore, the exploration of family data for the same population was intended, same as the determination of the single exclusion chance for non-fathers (SECNF).