Effect of melanin reduction by extracts from Ulmus davidiana by supercritical fluid extraction

Nowadays, many people prefer products containing functional substances extracted by using harmless extraction methods from environmentally friendly substances. In addition, according to the aging of the population, it consumes a lot in external parts regardless of gender. Accordingly, in this study, we would like to check the whitening function by applying extracts from Ulmus davidiana by supercritical fluid extraction method to melanocytes (B16F10) cells in order to utilize it as a functional material. Here, USCF was extracted from Ulmus davidiana branch (with barks) by supercritical extraction fluid and USCFR was extracted with 60% alcohol at room temperature and filtered from the residues acquired after supercritical extraction. USCFREA was fractionated with ethyl acetate, and the ethyl acetate layer extract was concentrated under vacuum concentration and freeze-drying to obtain final product. First, it was confirmed that the optimal concentration of DMSO for melanocytes was 1% or less. In addition, by using the extracts from the Ulmus davidiana, the melanin content for extracellular and intracellular was checked, and the best effect of melanin treatment was confirmed in sample 2 treated with 50 µg.