丹参酮IIA对自发性高血压大鼠左心室谷胱甘肽-s-转移酶μ2表达的影响

AIM: To observe the effects of tanshinone ⅡA on the expression of glutathione-s-transferase μ2 (GSTμ2) in spontaneously hypertensive rats, and to investigate the mechanism of the reversal of left ventricular hypertrophy by tanshinone ⅡA. METHODS: There are 3 groups in the experiment: control, spontaneously hypertensive rats (SHR), SHR treated by tanshinone ⅡA. At the endpoint of the experiment, hemodynamic parameters were measured. Then the rats were sacrificed, body weight (BW) and left ventricular weight (LVW) were measured, and the LVW/BW ratios were calculated. The oxidative fluorescence dye dihydroethidium was determined by laser confocal fluorescent microscopy to evaluate in situ O2(superscript •-) generation in the left ventricles. The expression of GSTμ2 was investigated by western blotting. RESULTS: Compared with control, the systolic blood pressure and LVW to BW ratios of SHR elevated markedly, the left ventricular function was decreased. The left ventricular hypertrophy was significantly reversed by tanshinone Ⅱ A treatment, but tanshinone Ⅱ A didn't decrease the systolic blood pressure of SHR. O2(superscript •-) generation increased in SHR significantly, and it was decreased by tanshinone Ⅱ A. The expression and activities of GSTμ2 in left ventricular significantly decreased in SUR, however that was reversed by tanshinone Ⅱ A. CONCLUSION: Tanshinone Ⅱ A can significantly reverse the left ventricular hypertrophy in SFTR, that might be associated with up-regulation of GSTμ2, decrease of O2(superscript •-) and elimination of oxidative stress.