Diagnostic potential of serum exosomal colorectal neoplasia differentially expressed long non-coding RNA (CRNDE-p) and microRNA-217 expression in colorectal carcinoma

// Bo Yu 1, 2, * , Qiong Du 1, 2, * , Huan Li 1, 2 , Hong-Yue Liu 1, 2 , Xuan Ye 1, 2 , Bin Zhu 1, 2 , Qing Zhai 1, 2 and Xin-Xiang Li 1, 3 1 Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China 2 Department of Pharmacy, Fudan University Shanghai Cancer Center, Shanghai 200032, China 3 Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai 200032, China * These authors contributed equally to this work Correspondence to: Qing Zhai, email: zhaiqing63@126.com Xin-Xiang Li, email: lxx1149@163.com Keywords: colorectal carcinoma, exosome, CRNDE-p, miR-217 Received: March 01, 2017      Accepted: July 12, 2017      Published: July 20, 2017 ABSTRACT In this study, we investigated the diagnostic potential of serum exosomal colorectal neoplasia differentially expressed (CRNDE-p) long coding RNA and microRNA-217 in colorectal carcinoma (CRC). We detected high CRNDE-p and low miR-217 levels in exosomes released by multiple CRC cell lines into culture media as well as in sera from CRC xenograft mice and CRC patients. Conversely, we observed lower CRNDE-p and higher miR-217 levels in serum exosomes from post-chemotherapy than from pre-chemotherapy patient samples. The area under curve (AUC) value for the serum exosomal CRNDE-p and miR-217 combination was higher than CRNDE-p or miR-217 alone. Moreover, high CRNDE-p and low miR-217 serum exosomal levels correlated with advanced clinical stages (III/IV), tumor classification (T3/T4), and lymph node or distant metastasis. Thus combined evaluation of serum exosomal CRNDE-p and miR-217 levels show diagnostic and prognostic potential for CRC patients.