Isolation and Expression Analysis of a Putative CIPK Gene in Halophyte Sesuvium portulacastrumand L.

Calcium plays a significant role in intracellular signaling. Calcineurin B-like proteins (CBLs) and their target proteins, CBL-interacting protein kinases (CIPKs) have emerged a calcium network responsing to stresses in plants. In this study, we cloned a CIPK gene, SpCIPK8, from the halophyte plant Sesuvium portulacastrumand L. Bioinformation analysis showed that the cDNA of SpCIPK8 was 1344 bp and encoded 447 amino acids with a molecular weight of 50.72 KD and an isoelectric point of 7.19. One transmembrane domain exists in this hydrophilic protein. Phylogenetic analysis showed that SpCIPK8 was similar with AtCIPK8 in Arabidopsis.The expression level of SpCIPK8 in stems was three times higher than that in roots and the level in leaves was two times higher than that in roots.