PENGARUH PEMBERIAN EKSTRAK BUAH DELIMA TERSTANDAR 40% ELLAGIC ACID SECARA TOPIKAL TERHADAP KEPADATAN KOLAGEN LUKA INSISI PADA TIKUS PUTIH (Rattus norvegicus)

The aim of this research was to determined the effect of pomegranate fruit extract (PFE) standardized 40% ellagic acid ointment to collagen density on incision wound in rat. Twenty five rats (Rattus norvegicus) with 60-90 day ages and 150-180 grams average of body weight were divided into five groups (P0, P1, P2, P3, and P4). P0 (control -) was not treated , P1 (control +) was treated with 10% povidone iodine , P2 was treated with PFE 40% ellagic acid 2,5%, P3 was treated with PFE 40% ellagic acid 5%, and P4 was treated with PFE 40% ellagic acid 7,5%. Treatment had been given directly on incision area topically for fourteen days. The data of histopatological appereance were analyzed with Kruskall-Wallis and continued with Mann-Whitney. Result showed there were significant (p<0,05) different between treatment group. Highest collagen density incision wound occurred in P4 significantly different with other group (p<0,05), giving PFE 7,5% increasing collagen density into score 4-5. Based on the result that the average increasing of collagen density is on PFE 40% ellagic acid treatment, more high PFE 40% ellagic acid concentration more higher the compounds of flavonoid, saponin, alkaloid, tannin, anthocyandin that contains antibiotic, antiseptic, antioxidant. Ellagic acid when combined with other compositions of pomegranate also serves as a powerful antioxidants specifically anthocyanidin, is an antioxidants that can help improve blood vessels. This research concluded that pomegranate fruit extract (PFE) 40% ellagic acid ointment concentration 7,5% can increased significantly collagen density on incision wound in rat.