РОЛЬ РЕДОКС-СТАТУСА И ОКИСЛИТЕЛЬНОЙ МОДИФИКАЦИИ БЕЛКОВ В РЕАЛИЗАЦИИ АПОПТОЗА ЛИМФОЦИТОВ КРОВИ ЧЕЛОВЕКА В НОРМЕ И ПРИ ЭКСПЕРИМЕНТАЛЬНОМ ОКИСЛИТЕЛЬНОМ СТРЕССЕ

The article presents the data on the level of reactive oxygen species, irreversible and reversible oxidative modification of proteins, the state of glutathione system components, activity of catalase, and implementation of apoptosis in intact blood lymphocytes. By modulating oxidative stress in vitro, we studied the role of hydrogen peroxide in the final concentration of 0.5 mM in apoptosis implementation. We also evaluated the contribution of the glutathione system components and irreversible and reversible oxidative modification of proteins to molecular mechanisms of apoptosis in blood lymphocytes. We showed that apoptotic death in blood lymphocytes under experimental oxidative stress was characterized by activation of protein glutathionylation and carbonylation. The obtained findings prove that protein molecules are potential redox-dependent targets for regulating cell metabolism in blood lymphocytes.