Perfusion machine for hypothermic pulsatile preservation of rat kidneys. Use in PUVA treatment of allogenic kidney transplants

A machine for continuous hypothermic pulsatile perfusion preservation of rat kidneys is described in detail. The use of longwave ultraviolet irradiation during hypothermic pulsatile kidney perfusion using Euro-Collins solution containing 8-MOP did not prolong graft survival compared with untreated controls. Systemic application of 8-MOP to the kidney donor may be necessary for effective action of the combined photochemical treatment as a method of immunoalteration.