Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone.
2012
The epidemic community-associated methicillin-resistant clone
Staphylococcus aureus USA300 is a major source of skin and soft
tissue infections and involves strains with a diverse set of
resistance genes. In this study, we report efficient
transduction of penicillinase and tetracycline resistance
plasmids by bacteriophages phi80alpha and phiJB between
clinical isolates belonging to the USA300 clone. High
transduction frequencies (10-5 to 10-6 CFU/PFU) were observed
using phages propagated on donor strains as well as prophages
induced from donors by ultraviolet light. Quantitative
real-time PCR was employed to detect penicillinase plasmids in
transducing phage particles and determine the ratio of
transducing particles in phage lysates to infectious phage
particles (determined as approximately 1:1700). Successful
transfer of plasmids between strains in USA300 clone proves
transduction is an effective mechanism for spreading plasmids
within the clone. Such events contribute to its evolution and
to emergence of new multiple drug-resistant strains of this
successful clone.
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