Separation of spermatozoa with a combination of pinched flow fraction and tangential filtration

We demonstrate a pinched flow tangential filtration method to sort spermatozoa from larger particles with a spermatozoa collection efficiency of 94±2% and a separation efficiency of 100%. In conventional pinched flow fractionation (PFF), an observed tumbling-like rotation of spermatozoa complicates their separation from other articles. The new design circumvents this effect.