PCR Amplification of Sox Gene of R.nigromaculata Using Two Pairs of Primers

The Sox gene of R.nigromaculata was amplified using two pairs of primers which specially amplify the conservation sequence of human HMG-box gene.Results show that: 1) The Sox gene exists in both female and male R.nigromaculata,and both the lengths of the fragments of PCR products are 220 bp.It shows that there is no difference in Sox gene between male and female R.nigromaculata;2) Both pairs of the primers have the same products.We can see the evolution of Sox gene.