DESIGN, SYNTHESIS, AND CHARACTERIZATION OF A NOVEL HEXA-AZACYCLOPHANE AND INTERACTIONS WITH D(CGCA3T3GCG)2, CTDNA AND T4DNA

Abstract A novel polyazacyclophane hexa-amine ( 1 ) has been designed, synthesized and characterized by X-ray crystallography, 1 H NMR and fluorescence spectroscopy. 1 has the structural shape and disposition of charges complementary to the major groove of B-DNA. Acid dissociation constants for the protonated 1 were pK a1 (D) = 4.8 and pK a2 (D) = 8.5. Equilibrium constants for the binding of 1 to ctDNA and T4 DNA were 1.2 × 10 5 and 1.8 × 10 6 , respectively.