Cloning of the Gene GH18Rorf392 Containing 26S rRNA Sequence from Cotton Restorer line

Y18R×P30A hybrid F1 generation then inbred,so judge the fertility according to stamen morphological character.Total RNA were extracted from young buds of 3～5 days from fertile and sterile cotton,respectively.EST sequence from cotton restorer line was obtained through suppression subtractive hybridization.Designing primers according to the EST sequence,then doing rapid amplification cDNA ends,so full length cDNA sequence was obtained.The gene was named by GH18Rorf392 because it came from Gossypium hirsutum restorer line Y18R and codes 392 amino acids.The 5' end of GH18Rorf392 is a new sequence and its 3' end contains 26S rRNA partial sequence.This gene can be used to study cotton fertility associated function.