Additional file 5: Figure S5. of Roles of acid-extruding ion transporters in regulation of breast cancer cell growth in a 3-dimensional microenvironment

Stability of transient siRNA-mediated knockdown of NHE1 in MDA-MB-231 cells. A. MDA-MB-231 cells were transfected with 100 nM siNHE1. 48 h after transfection, cells were reseeded and the stability of the knockdown in 2D culture was monitored for 120 h. Left panel show Western blots and right panel show quantifications of band intensities normalized to corresponding Mock control. B: Western blots showing CRISPR/Cas9-mediated knockout (KO) of NHE1 in MCF-7 and MDA-MB-231 cells. WT: wild-type. (PDF 135 kb)