In vitro multiplication of nodal segments of "Murici" (Byrsonimacydoniifolia A. Juss.): the use of growth regulators and photoautotrophic stimulation.

Micropropagation is an alternative method for the in vitro cultivation of “murici” (Byrsonimacydoniifolia A. Juss.), a plant from the Cerrado (Brazilian savanna) that has a high agronomic potential and is grown for its fruit but has limitations in terms of its propagation. Thus, the objective of the present study was to evaluate the use of growth regulators and photoautotrophy stimulation, testing different ways to seal the explant test tube and the use of sucrose for the in vitro cultivation of murici. In assay I, the medium was supplemented with BAP at concentrations of 0.0, 11.1, 22.2 and 44.4 µM and NAA at concentrations of 0.0, 6.71, 13.43 and 26.85 µM. In assay II, 4.9 µM IBA was added to the culture medium, with or without sucrose, with the use of 3 different seals: a plastic cap, plastic film and cotton plug. After 60 days of cultivation, the following traits were evaluated: the callus percentage, leaf number, shoot length and length and number of roots. It was determined that the concentrations of 11.1 µM BAP and 13.43 µM NAA were the most suitable. The type of seal did not influence the growth of B. cydoniifoliaA. Juss., but the medium supplemented with sucrose was effective for the growth of this species.