Comparative analysis of different methods for isolation of mesenchymal stem cells in mice

The present study was based on isolation and quantification of umbilical cord-derived mesenchymal stem cells (UC-MSCs) and adipose derived mesenchymal stem cells (AD-MSCs) from Swiss Albino mice. Umbilical cord and adipose tissues were collected from foetus and abdomen of mice respectively. Isolation of AD-MSCs and UC-MSCs from the collected tissues was done by collagenase type-I and trypsin-EDTA digestion. After processing the isolated cell pellet was seeded in a six well plate with fresh media containing DMEM Nutrient Mix F12, 10% FBS and 1% antibiotic antimycotic solution and incubated in humidified CO2 (5%) incubator at 37 °C till 70-80% confluence. Then the cells were collected for quantification and viability assessment by Neubauer’s chamber under microscope using trypan blue stain. Trypsinization of both tissue yielded less number of cells than collagenase treatment. But trypsinization provided higher viable cells as compared to collagenase treatment. Hence, trypsin-EDTA can be used for analytical study, where the cell yield is of lesser importance.