Enzymatic conversion of 5α-cholest-8(14)-en-3β-ol to 5α-cholesta-8, 14-dien-3β-ol

[3α-3H]-Cholest-8(14)-en-3β-ol was incubated with a 10,000 x g supernatant fraction of a rat liver homogenate. The enzymatic formation of labeled cholesta-8, 14-dien-3β-ol was indicated by the results of studies employing column chromatographic and cocrystallization techniques.