Disc electrophoresis of lactate dehydrogenase isoenzymes

Abstract The original method of disc electrophoresis permitted the localization of only 4 of the 5 lactate dehydrogenase isoenzymes. Conjecture as to the location of one of these is now settled in that it is demonstrated that the lactate dehydrogenase-5 does not migrate through the stacking gel. This was shown for human serum and liver. All 5 isoenzymes migrate into the separating gel by our earlier method. To obtain desirable separations of these for quantitation, it is necessary to use an upper gel of the same composition as the separating gel, to control the geometry of the gels, and to apply an optimal amount of sucrose with the sample.