Effect of Metformin and Detorsion Treatment on Serum Anti-Müllerian Hormone Levels and Ovarian Histopathology in a Rat Ovarian Torsion Model.

Backround/aim: Adnexal torsion is a common gynecological emergency, and considered to be a problem mostly in reproductive-age women.To evaluate the effect of metformin and detorsion treatment on reducing ovarian reserve in an ovarian torsion model. MATERIALS AND METHODS: Twenty-four nonpregnant, Wistar Hannover rats were included in the study. Animals were divided into 3 groups: the control group, the detorsion only group, and the metformin + detorsion group. The first group received only laparotomy. In the second group, ovaries were fixed to the abdominal wall after performing 360 degrees ovarian torsion, followed by detorsion after a 3-hour period of ischemia. The third group underwent the same torsion and detorsion procedures as the second group, and received 50 mg/kg metformin by gavage for 14 days. Ovarian damage scores, follicle counts, and AMH levels were evaluated. RESULTS: The total damage score was significantly increased in the detorsion only group compared to the metformin+detorsion and control groups. Pre-operative/post-operative AMH decreases were statistically significant in negative direction in the detorsion only group when compared to the metformin+detorsion and control groups (p=0.001). CONCLUSION: Metformin+detorsion treatment may be effective in protecting the ovarian reserve after ovarian torsion.