Laser speckle contrast imaging system using nanosecond pulse laser source.

SIGNIFICANCE Nanosecond-pulsed laser has proven to be used to obtain the velocity of blood using the speckle contrast method. Without the scanning time, it has potential for achieving fast two-dimensional blood flow images in a photoacoustic imaging system with the same pulsed laser. AIM Our study aimed to evaluate the qualities of regional cerebral blood flow (rCBF) obtained in a laser speckle contrast imaging (LSCI) system using continuous wave (cw) and nanosecond pulse laser sources. APPROACH First, a LSCI system consisting of a cw laser with a wavelength of 632.8 nm and a cw laser/nanosecond pulse laser with a wavelength of 532 nm was developed. This system was used to obtain rCBF images of mouse in vivo with two different laser sources. RESULTS Continuous wave lasers (532 and 632.8 nm) show different imaging characteristics for rCBF imaging. The rCBF images obtained using 532-nm nanosecond pulse laser showed higher resolution than those using 532-nm cw laser. There was no significant difference in the results using nanosecond pulse laser among various pulse widths or repetition rates. CONCLUSIONS It is proved that a nanosecond pulse laser could be used for LSCI.