The study of hyoscyamine in oxidative stress of liver cells in male rat

Background and aims: Increased production of free radicals by endogenous systems and exogenous sources in cells leads to oxidative stress, which damages to the cells of various organs. Hyoscyamine is one of the important tropane alkaloid isolated from some Solanaceous species used to traditional medicine that they are used for their analgesic, anti-inflammatory, antipyretic, and anticonvulsant activities. The antioxidant and antiglycation properties of tropane alkaloids may represent a role in dealing with oxidative stress. The aim of this study was to investigate the antioxidant and antiglycation effects of hyoscyamine component on the liver cells in male rats. Methods: In this experimental- laboratory study, liver cells were isolated from male Sprague–Dawley rats. The cells cultured under standard conditions. Various concentrations of hyoscyamine (0-32 µM) were treated on rat liver cells. Then, the activity of glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase (CAT) as well as glyoxal and 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition were measured by spectrophotometry. High-performance liquid chromatography (HPLC) was performed for measuring malondialdehyde (MDA) in liver cells. Results: CAT, SOD and GPX enzyme activities increased as the concentration of hyoscyamine increased. DPPH showed a strong inhibition on reactive oxygen species generation compared to control group. The amount of SOD, CAT and GPX enzyme activities in 8 micromolar concentration of hyoscyamine compared with the control group significantly increased as 10.33 and 8.6 and 6.3 units (P<0.05). Also, hyoscyamine (4µM) reduced the amount of MDA, glyoxylate and DPPH compared to the control group as 1.94, 2.26, and 2.33 times (P<0.05). Conclusion: Our findings indicated that hyoscyamine had considerable antioxidant and antiglycation activities on rat liver cells. This compound protects liver cells against the damaging effects of free radicals. The effects of this compound for the treatment of diseases associated with oxidative stress would be useful in the future.