Dynamic Observation of Zinc alpha2 Glycoprotein in Mice of Non-alcoholic Fatty Liver Disease

Objective To establish a nonalcoholic fatty liver disease model induced by high fat diet and observe the dynamic changes of Zinc alpha2 glycoprotein.Methods All of 48 C57BL/6 mice were randomly divided into high fat diet(HFD) group and standard diet(SD)group.The serum IL-6,IL-8,TNF-α,IL-1β and ZAG were assayed by ELISA kit at the 4th,8th,12th and 16th week.Hepatic triglyceride content was detected by ELISA kit.The liver tissues of mice were stained by HE.The mRNA expression of ZAG in liver was detected by real-time PCR.Results Compared with SD group,the level of serum TNF-α was significantly increased in HFD group from the 4th week(P0.05).From the 8th week,the weight gain,liver index,hepatic triglyceride content,IL-6,IL-8 and IL-1β were increased gradually(P0.05).The mRNA level of ZAG in liver of mice induced by HFD was gradually decreased from the 4th week(P0.05).From the 8th week,the serum ZAG in HFD group was decreased compared to SD group(P0.05).Conclusion The mouse model of NAFLD could be successfully established with high fat diet.The low expression level of ZAG in liver could be associated with the development of NAFLD.