Isolation, identification and bioactivity of steroids compounds from red algae Eucheuma cottonii petroleum ether fraction

More than 75 % of Indonesia is covered by the sea. Indonesia has abundant natural resources that have potential to be explored as new renewable bioactive compounds. One of the natural resources is red algae Eucheuma cottonii. The aim of this research was isolating, identifying and determining the bioactivity (toxicity and antioxidant activity) of steroids compounds in Red Algae Eucheuma cottonii. Red Algae Eucheuma cottoni was extracted by maceration using methanol. The methanol extract was hydrolyzed with 2 N of hydrochloric acid and then partitioned with petroleum ether. Petroleum ether fractions were identified by phytochemical test and LC-MS to know the types of steroids compound contained. Then, the steroids compound was separated with Preparative Thin Layer Chromatography (TLC) and Column Chromatography. The toxicity level and antioxidant assay of steroids isolates were determined by BSLT and DPPH method. According to phytochemical test and LC-APCI-MS, petroleum ether fraction of Red Algae Eucheuma cottoni contained steroids compounds such as cholesterol, β-sitosterol, campesterol and stigmasterol. LC50 value of isolates of petroleum ether fraction was 26.70 ppm (A1), 16.30 ppm (A3) and 22.03 ppm (A5). EC50 value of steroid isolates of petroleum ether fraction was 13.07 ppm (A1), 3.9.1010 ppm (A4) and 50.92 ppm (A5).