Effect of ginsenoside Rg1 on functional expression of human neural stem cells: a patch clamp study

Objective: To observe the effects of ginsenoside Rg1 on the functional expression of human neural stem cells(hNSCs).Method: The membrane electrophysiological properties and sodium and potassium ion channels in the hNSCs induced by Rg1 were analyzed using the whole-cell patch-clamp.Result: On the 7th day,the neuron-like cells derived from ginsenoside Rg1(20 mg·L-1)-induced NSCs show:① The resting membrane potential:(-45.70±2.63) mV,the membrane capacitance:(26.89±1.91) pF,the membrane input impedance:(877.51±20.44) MΩ(P0.05 compared with the control group,respectively);② The detection rate of inward sodium current which is rapidly activated and inactivated in voltage-dependence was 50%,and its average peak value was(711.48±158.03) pA(P0.05 compared with the control group);③The outward potassium currents were composed of rapidly activated and inactivated transient outward potassium current and delayed rectifier outward potassium current,and its average peak value was(1 070.42±177.18) pA(P0.05 compared with the control group).Conclusion: Ginsenoside Rg1 can promote the functional expression and maturity of hNSCs.