[Protective effect of astaxanthin against epididymal oxidative damagein rats with ornidazole-induced oligoasthenozoospermia].
2017
Objective: To investigate the improving effect of astaxanthin (AST) on the sperm quality of rats with ornidazole (ORN)-induced oligoasthenozoospermiaand its action mechanism. METHODS: Forty adult male SD rats were equally randomized into groups A (solvent control), B (low-dose ORN [400 mg/(kg·d)]), C (high-dose ORN [800 mg/(kg·d)]), D (low-dose ORN [400 mg/(kg·d)] + AST [20 mg/(kg·d)]), and E (high-dose ORN [800 mg/(kg·d)] + AST [20 mg/(kg·d)]), all treated intragastrically for3 weeks.After treatment, the epididymal tails ononeside was taken for determination of sperm concentration and activity, and the epididymideson the other side harvested for measurement of the activities of GSH-Px, GR, CAT and SOD and the MDA contentin the homogenate. RESULTS: Compared with group A, sperm motilityin the epididymal tail andGSH-Px and SOD activities in theepididymiswere markedly decreased while the MDAcontent significantlyincreased in group B (P<0.05), spermmotility and concentrationin the epididymal tail, testisindex, and the activities of GSH-Px, GR, CAT and SOD in the epididymis were remarkably reduced while theMDA contentsignificantly increased in group C(P<0.05). In comparison with group B, group D showed markedly increased sperm motility ([45.3±8.7]% vs [66.3±8.9]%, P<0.05) in the epididymal tail and SOD activity in the epididymis ([116.7±25.3] U/mg prot vs [146.1±23.8] U/mg prot, P<0.05), decreased MDA content([1.68±0.45] nmol/mg prot vs [1.19±0.42] nmol/mg prot, P<0.05).Compared with group C, group Eexhibited significant increases in the weight gained ([89.0±9.5] vs [99.9±4.1] %, P<0.05) and sperm motility ([17.9±3.5]% vs [27.3±5.3] %, P<0.05) but a decrease in the content of MDA ([2.03±0.30] nmol/mg prot vs [1.52±0.41] nmol/mg prot, P<0.05). CONCLUSIONS: AST can improve spermquality in rats with ORN-inducedoligoasthenozoospermia, which may be associated with its enhancing effect on the antioxidant capacity of the epididymis.
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