방선균이 생산하는 Cephalosporin 내성 병원성 Pseudomonas 에 유효한 항생물질

We isolated actinomycetes LAM-98-80 as strain producing an effective antibiotic for cephalosprin resistant pathogenic Pseudomonas sp. and identified as Streptomyces sp. LAM-98-80 from cultural and physiological characteristics. We investigated the optimal culture conditions for production of an antibiotic becoming effective for cephalosporin-resistant pathogenic Pseudomonas sp. It was found that 1.5% soluble starch and 1.0% yeast extract were good as carbon and nitrogen source, respectively. The production of antibiotic was also activated by 0.04% Mn^2+ as 80% degree. The optimum initial pH on production of antibiotic was pH 7.0. The culture condition for the maximal productivity of the antibiotic was at 30℃ for 5 days. The cephalosporin-resistant pathogenic Pseudomonas sp. as test bacteria was revealed to resist antibiotic of cepha families, but revealed to not resist those of β-lactam families, ampicillin and amoxicillin. Partial purified antibiotic was stable for the pH from 3 to 9, and was also stable when treated at 70℃ for 1 hour. This antbiotic was effective against all gram positive and negative bacteria but was not effective against molds and yeasts.