기내 감마선 조사에 의한 팔레높시스의 생육 및 감수성

The study was conducted to develop the method of making up new cultivars of phalaenopsis through the induction of mutation by in vitro irradiation with the gamma rays. While culturing the Phalaenopsis the material in PLB state at the stage of in vitro protocorm and seedlings in vitro about to be planted on outside place were treated with 20, 40, 60Gy of gamma rays. And, the ratio of the PLB necrosis, the degree of the sensitivities and variation for the gamma ray treatment in the M1 generation after the transplant of in vitro seedlings were measured and examined. The necrosis ratio at the in vitro protocorm stage was between 40.5% and 62.0% at the treatment with 20Gy gamma ray, 85.4% and 82.2% at the treatment with 40Gy gamma ray, and 84.0% and 94.2% at the treatment with 60Gy gamma ray, showing the optimal amount of the irradiation treatment is 20 Gy or below the level. Even though all the seedlings survived the in vitro treatment, the experiment showed the results that the higher the irradiation amount gets, the later the generation of a new leaf, and such negative phenomena as brown spots on the edge of a leaf or curling disease leafroll dialal was observed, producing new mutant forms with small flowers in size by making flower stalk suddenly out of season due to the irradiation of gamma rays. In the sensitivities of gamma rays in the flowering period of M1 generation by the irradiation quantity, the mini group with flower stalk length did not show a clear difference in comparison with the control group in terms of the amount in irradiation treatment, but in the group with long flower stalk showed that the higher the amount of the irradiation treatment, the shorter it gets. The number of the flowers is divided into KS413 group showing the increase in difference and KS4861 showing the decrease in difference, according to the amount of the irradiation treated with, depending on the group of cultivars publicly announced. The result has shown us that the higher the amount of the irradiation treated with, the smaller the length and width of a leaf, and that the length and width of a flower, a calyx, and a petal were al mostsimilar or a little bit smaller by the group of the cultivars publicly announced.