Blood group B-specific lectin of Plecoglossus altivelis (Ayu fish) eggs.

Abstract A lectin that agglutinates human blood group B erythrocytes but not blood group A and O erythrocytes was isolated from eggs of Ayu sweet fish ( Plecoglossus altivelis ). The lectin also agglutinates Ehrlich ascites carcinoma cells but not rat ascites hepatoma AH109 or rat sarcoma 150 cells tested. The lectin agglutination was most effectively inhibited by monosaccharides with the first type of configuration, i.e., L -rhamnose, L -mannose and L -lyxose at a concentration of 0.03 mM. The lectin agglutination was moderately inhibited by monosaccharides with the second type of configuration, i.e., D -galactose, D -fucose and D -galacturonic acid at a concentration of 0.4 mM. However, the agglutination was not inhibited by various other monosaccharides and oligosaccharides that have other types of configuration. The basis for an apparent B-specific hemagglutination may be due to the steric similarity of the C 2 and C 4 of the galactosyl series, the B-specific determinant, and the L -rhamnosyl series, which are the best inhibitors of the lectin activity. The lectin was affinity purified on an L -rhamnosyl-Sepharose column and was characterized as a homogeneous low molecular weight protein ( M r 14 000) with an abundance of hydrophobic amino acids and dicarboxylic amino acid.