The production of a human monoclonal antibody defining a split of HLA-DRw13 (DRw13b)

By use of the heterohybridoma technique we have produced a human monoclonal antibody (NDS40) which detects a split of HLA-DRwl 3 (DRwl3b) which is in linkage with HLA-DQwl. In addition, the antibody reacts with cells positive for HLA-DRw8 and DRwl 1, but does not react with the commonly found split of DRw1 3 (DRw1 3a) which is associated with DQwl. NDS40 is cytotoxic and is of the lambda IgM class.